COMBINED EFFECT OF TOTAL AMMONIA AND TEMPERATURE ON GROWTH, HEMATOLOGICAL, BIOCHEMICAL PARAMETERS AND HISTOLOGICAL INDICES FOR RED TILAPIA (Oreochromis sp.)

Authors

1 Dept. Fish Res. and Aquacul., Fac. Environ. Agric. Sci., Arish Univ., Egypt.

2 Fac. Agric., Banha Univ., Egypt.

Abstract

This study aims to evaluate different levels of total ammonia, temperature and their combination on growth, feed utilization, hematological, biochemical parameters and histological indices in red tilapia (Oreochromis sp). Nine treatment which is combination of three levels of total ammonia (1.5, 3.0 and 4.5 ppm) and three different temperature degrees (24, 28 and 32oC) in a 3×3 factorial trial was designed for 56 days. Ten fish with an average initial body weight 11.1±0.27 g and an average initial body length 6.98±0.30 cm were distributed with two replicates. Analysis of variance showed significant differenc (P<0.05) of hematological parameters for RBCs, WBCs, Hb, Hct, PLT, MCH and MCHC among treatments. Additionally, biochemical parameters; glucose, uric acid, urea, albumin and globulin, and liver function activity were significantly different (P<0.05). The optimal activities of alanine and aspartate aminotransferase were obtained by fish of T5 and T1, respectively. For all treatments, the results of gill investigations, showed moderate degeneration of filaments with moderate deed of lining cells, shortening focal autolytic changes and focal congestion. The results of kidney histology showed marked interstitial edema, congestion with focal inflammatory infiltrate, mild vacuolar degeneration, focal edema and focal shrinkage for few glomeruli. The present findings suggest that interaction between total ammonia and temperature affect greatly fish health, physiological and histological status.

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