EFFECT OF EXPLANT TYPES AND CYTOKININ TYPES ON GROWTH OF BITTER AND SWEET ALMOND CUTTINGS DURING ESTABLISHMENT AND PROLIFERATION STAGES In vitro

Authors

1 Dept. Plant Prod., Fac. Environ. Agric. Sci., Suez Canal Univ., Arish, Egypt.

2 Inst. Genetic Eng. and Biotechnol., Sadat City, Monofia Univ., Egypt.

Abstract

Embryo, Shoot tip and one node cutting from bitter and sweet almond are excised; sterilized, and cultured on Murashige & Skoog (MS) medium. Also, different cytokinin types were evaluated. The obtained results showed that culturing of embryo on free MS medium was effective in enhancing establishment stage of bitter almond. However, sweet almond was enhanced by MS medium supplemented with BAP. Meanwhile, adding 1.0 mgl-1 BAP for bitter almond and 2.0 mgl-1 BAP for sweet almond encouraged the highest proliferation.

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