REGULATION OF STEVIA ORGANOGENESIS UNDER STRESS AND MICRO-CLIMATIC CONDITIONS

Authors

1 Cent. Lab. Agric. Climate, Agric. Res. Cent., Min. Agric. and Land Reclam., Egypt.

2 Dept. Plant Prod., Fac. Environ. Agric. Sci., Arish Univ., Egypt.

Abstract

This study aimed to evaluate the performance of Stevia (var. Spanti) to in vitro salinity and drought stresses as well as micro climatic conditions. Nodal segments were cultivated on B5 medium under (22±2ºC) temperature and light intensity (2500 LUX). Different concentrations from NaCl (0, 1000, 2000, 3000, 4000 and 5000 ppm) and mannitol (0, 2, 4, 6, 8 and 10 bar) were used to study salinity and drought stresses. Moreover, two different temperature degrees (18 and 22°C) with three levels of light intensity (1000, 2000 and 3000 LUX) were used to study the influence of temperature and light intensity on stevia development. The growth parameters values for both salinity and drought treatments were recorded highest values using 1000 ppm and recorded lowest values using 10 bar, respectively. The results showed that stevia plants under 22°C and 2000 LUX treatments showed the highest values for each of growth parameters and organic and mineral contents.

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