EFFECT OF SOME GROWTH REGULATORS AND GELLING AGENT ON MICROPROPAGATION OF Codiaeum varigatum

Soliman, Dina A.; Hassan, Hany M.S.; Ali, Mohamed A.M.

doi:10.21608/sinjas.2016.78643

EFFECT OF SOME GROWTH REGULATORS AND GELLING AGENT ON MICROPROPAGATION OF Codiaeum varigatum

Authors

Dept. Plant Prod., Fac. Environ. Agric. Sci., Arish Univ., Egypt.

10.21608/sinjas.2016.78643

Abstract

This study was carried out in Plant Tissue Culture Laboratory of the Faculty of Environ. Agric. Sci. El-Arish, North Sinai, Suez Canal University during the period from 2012 to 2015. The present studies were undertaken to look for additional cheap and easily available substitutes of agar to be used in tissue culture media. In order to enhance the micropropagation efficiency of Codiaeum variagatum different concentrations (0, 1, 2, 4, 8 and 16 mg l^-1) of benzyle adenine (BA) or Kinetin (Kin) were investigated during establishment stage. Results indicated that kin at 4 mgl^-1 was the best treatment during this stage. During multiplication stage different concentrations (0.0, 0.1, 0.2 and 0.4 mgl^-1) of IAA or NAA combined with 4 mgl^-1 Kin were tested. Recorded data referred that 0.4 mgl^-1 NAA combined with 4 mgl^-1 Kin gave the best values for shoots proliferation and growth. Toward searching for low cost gelling agent could be used as alternative for high price agar, different concentrations (10, 20, and 30 gl^-1) of guar seed powder or different combinations between locust bean (LB) and agar (6 agar + 2 LB, 4 agar + 4 LB, 2 agar+ 6 LB, 2 agar or 8 agar, gl^-1) were used as gelling agents. Recorded data indicated that 4 gl^-1 agar +4 gl^{-1 LB gave the maximum values of shoot proliferation and growth. Since the produced shoots from multiplication stage were quite short, different concentrations (0.0, 1.0, 2.0 and 4 mgl-1}) of GA₃ combined with 4 mgl^-1 Kin + 0.4 mgl^-1 NAA were examined. Addition of GA₃ at 4 mgl^-1 combined with the above mentioned growth regulators proved to be the treatment in this regard. In order to enhance root indication on obtained shoots bases different concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg l^-1) of IBA, activated charcoal (0.0 and 1.0 mgl^-1) and MS strength (full and half) were evaluated .Recorded data stated that there were no significant differences among some of applied treatments, but the highest number of roots/explant and root length were obtained when shoots were cultured on full MS supplemented with 1.5 mgl^-1 IBA without activated charcoal. Produced plantlets were successfully acclimatized (100% survivability) in vermiculite and peat (1:1, V/V) medium.

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